Optimised cord blood sample selection for small‑scale CD34+ cell immunomagnetic isolation

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Optimised cord blood sample selection for small‑scale CD34+ cell immunomagnetic isolation.

Haematopoietic stem cells (HSCs) are defined as multipotential cells, capable of self-renewal and reconstituting in vivo the haematopoietic compartment. The CD34 antigen is considered an important HSCs marker in humans. Immunomagnetic isolation, by targeting CD34 antigen, is widely used for human HSC separation. This method allows the enrichment of human HSCs that are present at low frequencies...

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Cord blood is a sample of blood taken from a newborn baby's umbilical cord.Cord blood is collected because it is rich in stem cells content, such as hematopoietic cells, which can be used to treat hematopoietic and genetic disorders. Cord Blood Transplantation has been used in the treatment of over 80 diseases, including leukemia, lymphoma and anemia. Advantages of Cord Blood Treatment: • ...

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Isolation of fetal nucleated red blood cell from maternal blood using immunomagnetic beads for prenatal diagnosis.

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CD34 enrichment of umbilical cord blood.

OBJECTIVE We describe the relation between the clamping time and blood volume collected, plus two enrichment systems for CD34+ stem cells from umbilical cord blood, to achieve an excellent recovery of cells with a high proliferative ability and bone marrow reconstitution. METHOD After cord blood collection by an obstetrician, stem cell purification has been performed either with a combination...

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CD34+ stem cells from umbilical cord blood

We describe the relation between umbilical cord clamping time and two different enrichment system of CD34(+) stem cells from umbilical cord blood with the proliferative ability and bone marrow reconstitution of the stem cells obtained. After an obstetrician performed the cord blood collection, the purification of stem cells was performed either with a combination of monoclonal antibodies (negat...

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ژورنال

عنوان ژورنال: Molecular Medicine Reports

سال: 2011

ISSN: 1791-2997,1791-3004

DOI: 10.3892/mmr.2011.733